Reduction of the Formyl Group of Zinc Pheophorbide b in vitro and in vivo: a Model for the Chlorophyll b to a Transformation
نویسندگان
چکیده
Verena Scheumann, Michael Helfrich, Siegrid Schoch and Wolfhart Rüdiger Botanisches Institut der Universität München, Menzinger Straße 67, D-80638 München Z. Naturforsch. 51c, 185-194 (1996); received November 17/Decem ber 18, 1995 Avena sativa L., Infiltration into Etiolated Leaves, Chemical Reduction, Cyanoborohydride, Phytylation The chemical reduction of the formyl group of pheophorbide b with sodium cyanoborohy dride in methanol leads to 7 ‘-methoxyand 71-hydroxy-pheophorbide a. The same reaction with zinc pheophorbide b yields in addition zinc pheophorbide a. This was characterized by mass and 'H-NMR spectroscopy. Infiltration of zinc pheophorbides a and b and of zinc 71hydroxy-pheophorbide a into etiolated oat leaves yielded phytylated products. The best yield in the esterification was obtained with 7 1-hydroxy-pheophorbide a. Analysis of the products revealed the formation of zinc pheophytin a from all infiltrated compounds. The significance for the transformation of chlorophyll b into chlorophyll a is discussed.
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